Two-dimensional peptide mapping of protease-digested proteins from two-dimensional electrophoresis gel spots

Conference · Tue Dec 31 23:00:00 EST 1985

OSTI ID:6899211

Zhang, J S; Giometti, C S; Tollaksen, S L

A simple procedure is described to extract proteins from polyacrylamide gels and subsequently separate them after partial proteolysis. This method is useful for the biochemical characterization of individual proteins in two-dimensional gel patterns and for the identification of both similarities and differences between a pair of related protein spots. Because the number of gel spots to be extracted and the extraction volume can be adjusted in this procedure, differences in the primary structure of both abundant and minor proteins can be investigated. By extracting protein from 10 or more gel spots, enough protein should be obtained to allow amino acid sequencing of peptides that carry significant amino acid differences. 7 refs., 1 fig., 1 tab.

• Research Organization: Argonne National Lab., IL (USA)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6899211
• Report Number(s): CONF-8609239-2; ON: DE87004943
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105 -- Separation Procedures
440300 -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CHROMATOGRAPHY
DESIGN
DIALYSIS
ELECTRODIALYSIS
ELECTROPHORESIS
ESTERS
EXTRACTION APPARATUSES
EXTRACTION CHROMATOGRAPHY
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PEPTIDES
POLYACRYLATES
POLYMERS
POLYVINYLS
PROTEINS
SEPARATION EQUIPMENT
SEPARATION PROCESSES