Surfactant phospholipid recycling in black and beige mice: kinetics
To determine details of kinetics and pool sizes of pulmonary surfactant the authors measured specific activities of disaturated phosphatidylcholine (DSPC) in 96 mice of each strain from 1-100h after administration of /sup 3/H-glycerol. DSPC was purified from lamellar bodies (LB) and alveolar lavage (AF) of each mouse. These data were used to construct turnover curves which were analyzed by a nonlinear least squares program to obtain best fit rate constants and relative pool sizes for a 3 compartment model with recycling between AF and LB. LB's were pure by ultrastructural and biochemical tests; /sup 14/C DSPC was added as internal standard for quantitation of AF DSPC pool size. Compartment sizes, ..mu..g phosphorus (P), were for black mice, LB 5.9; AF 9.2, and for beige mice, LB 15.0; AF 5.4. The large LB DSPC pool in beige mice, which is known to be associated with large abnormal LB's, could be due to the 5-fold increased uptake of DSPC from AF to LB. Equally possible, and more likely, is that much of their large LB DSPC pool is not secreted and does not participate in surfactant turnover.
- Research Organization:
- Hines VA Hospital, IL
- OSTI ID:
- 6898909
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
BODY
CARBON 14 COMPOUNDS
ESTERS
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LAVAGE
LEAST SQUARE FIT
LIPIDS
LUNGS
MAMMALS
MAXIMUM-LIKELIHOOD FIT
MICE
NUMERICAL SOLUTION
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PURIFICATION
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SURFACTANTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES