Zeatin-binding proteins in barley leaves

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Dokl. Bot. Sci. (Engl. Transl.); (United States)

OSTI ID:6898390

Romanov, G A; Kulaeva, O N; Taryan, V Y

Highly labelled tritium-zeatin was used in the work to clarify for the first time a protein factor that is present in cytokinin-sensitive vegetative organs of plants (barley leaves) and which possesses the properties of a cytokinin receptor. Aliquots of tritium-zeatin were mixed with a solution of protein and incubated for several hours in buffer. Following incubation, protein was precipitated by ammonium sulfate at 90% of saturation, and radioactivity of the precipitate was checked in a dioxane scintillator with an efficiency of about 35%. It is shown that the characteristics of interaction of the clarified specific protein sites with cytokinins in regard to a number of criteria correspond to the characteristics expected of receptors of these phytohormones.

• Research Organization: K.A. Timiryazev Institute of Plant Physiology, Acad of Sci., Moscow
• OSTI ID: 6898390
• Journal Information: Dokl. Bot. Sci. (Engl. Transl.); (United States), Journal Name: Dokl. Bot. Sci. (Engl. Transl.); (United States) Vol. 286:3; ISSN DKBSB
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BARLEY
BIOCHEMICAL REACTION KINETICS
CEREALS
COMPLEXOMETRY
GAS SCINTILLATION DETECTORS
GRASS
HORMONES
ISOTOPE APPLICATIONS
KINETICS
KININS
LABELLED COMPOUNDS
LABELLING
LEAVES
MEASURING INSTRUMENTS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
POLYPEPTIDES
PRECIPITATION
PROTEINS
RADIATION DETECTORS
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
SCINTILLATION COUNTERS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS