In vitro biodegradation of steranes
- Sanofi Elf Bio-Recherches - BP, Labege (France)
- Elf Aquitaine - SNEA(P)
The purpose of this paper is to report reproducible results on the in vitro biodegradation of steranes in various crude oils. 73 pure strains including Pseudomonadacea (33) and Actinomycetaceae (40) have been screened in order to test their capability to degrade steranes contained in total alkanes isolated from various crudes. Biodegradation of steranes has been observed with 7 strains belonging to Nocardia and Arthrobacter genera. 5{alpha}(H), 14{alpha}(H), 17{alpha}(H) and 5{alpha}(H), 14{beta}(H), 17{beta}(H) Steranes with the 20R configuration were degraded under reproducible laboratory conditions. Biodegradation of the sterane mixtures isolated from crude oils followed W. Seiferts rules established on the basis of geological observations. 5{alpha}(H), 14{alpha}(H), 17{alpha}(H) C{sub 27}-Steranes with the 20R configuration are degraded first and ends with the 5{alpha}(H), 14{alpha}(H), 17{alpha}(H) C{sub 29}steranes. Then 5{alpha}9h0, 14{beta}(H), and 17{beta}(H) steranes are attacked starting with the 20R configuration. Limited alteration of Tm and Ts terpane has also been observed.
- OSTI ID:
- 6894412
- Report Number(s):
- CONF-8904126-; CODEN: ACPCA
- Journal Information:
- American Chemical Society, Division of Petroleum Chemistry, Preprints; (USA), Vol. 34:1; Conference: Biomarkers in petroleum: memorial symposium for Wolfgang K. Seifert, Dallas, TX (USA), 9-14 Apr 1989; ISSN 0569-3799
- Country of Publication:
- United States
- Language:
- English
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