Crystalline ricin D, a toxic anti-tumor lectin from seeds of Ricinus communis
A toxic lectin, ricin D, present in the seeds of Ricinus communis has been purified and crystallized in a form suitable for high resolution crystallographic structure studies. This protein is different from a previously found form of ricin (also present in the same seeds), the only ricin for which a preliminary x-ray investigation has been reported so far. Ricin D crystallizes from an aqueous solution in an orthorhombic unit cell of symmetry P2/sub 1/2/sub 1/2/sub 1/ and a = 79.0, b = 114.7, and c = 72.8 A. The asymmetric unit contains one molecule with an average molecular weight of 62,400. The crystal is fairly stable to x-radiation and has a water content of approximately 54% by volume. It appears to comprise two closely related species of proteins, the major species corresponding to ricin D and the other presumably corresponding to a deamidation product of ricin D. The two species have nearly identical molecular size and amino acid compositions, but different charges.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 6893965
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 253:6; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560110 -- Radiation Effects on Biochemicals-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMISTRY
CASTOR
CHEMICAL COMPOSITION
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CRYSTALLIZATION
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MEDICINAL PLANTS
ORGANIC COMPOUNDS
PHASE TRANSFORMATIONS
PLANTS
PROTEINS
PURIFICATION
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
SEEDS
X RADIATION