Effect of medium modification and selected precursors on sterol production by short-term callus cultures of Euphorbia tirucalli

Journal Article · Sun Dec 31 23:00:00 EST 1978 · J. Nat. Prod.; (United States)

DOI:https://doi.org/10.1021/np50006a011· OSTI ID:6890594

Biesboer, D D; Mahlberg, P G

Latex from E. Tirucalli, a potential rubber source, contains steroidal alcohols that are high in energy and thus of value in biomass conversion to fuels. Euphol was present in large amounts in the latex, but tirucallol predominated in greater quantities in explants and callus indicating synthesis and/or accumulation of tirucallol by cells other than the laticifer cell. Sterol production was significantly enhanced by certain nutrient media, as well as indole-3-acetic acid, and depressed by benzyladenine. Precursor stimulation of product synthesis was successful only with squalene, which promoted sterol production at 1.0 mg/liter but inhibited cell growth at higher concentrations. DL-mevalonic acid and lanosterol promoted neither growth nor sterol production. DL-(214C) mevalonate was used to confirm the biosynthesis of sterols in both latex and callus cultures.

• Research Organization: Indiana University, Bloomington, IN 47401, USA
• OSTI ID: 6890594
• Journal Information: J. Nat. Prod.; (United States), Journal Name: J. Nat. Prod.; (United States) Vol. 42:6; ISSN JNPRD
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
BIOSYNTHESIS
CELL CULTURES
CLONING
ELASTOMERS
EUPHORBIA
HYDROCARBONS
HYDROXY COMPOUNDS
LATEX
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PLANTS
POLYENES
POLYMERS
PRECURSOR
RUBBERS
SQUALENE
STEROIDS
STEROLS
SYNTHESIS
TERPENES