Comparative studies on the structure of human adenovirus genomes 4, 7 and 21. Annual progress report 1 Jul 79-20 Jan 80

Technical Report · Fri Feb 01 00:00:00 EST 1980

OSTI ID:6886885

Padmanabhan, R

Adenovirus Type 7 (Greider Strain) was grown in human KB cells. The virions were purified by CsCl equilibrium centrifugation, the DNA was extracted and purified. The viral genome was analyzed by restriction endonucleases EcoRl, BamHI, SmaI, HpaI, BelI, BstEII and KpnI. The restriction endonuclease patterns of the viral DNA was compared with those of three Ad 7 subgroups. Patterns we obtained by cleaving Ad 7 with SalI, HpaI, Bam Hi and SmaI were identical to those obtained by cleaving Ad 7 with SalI, HpaI, Bam Hi and SmaI were identical to those obtained with strain 1058 and strain 55142 (Ad 7 vaccine strain). However, EcoRI pattern of Ad 7 (Greider) was different from strain 1058 and 55142 in that one site mapped at 85.4 units from the left end of the DNA is missing. DNA sequence at the inverted terminal repetition of Ad 7 DNA was analyzed in order to compare Ad 7 (Greider) with Ad 3 DNA, another member of 'weakly oncogenic' group (B). Ad 2 (add Ad 5) member of non-oncogenic group (C) and with Ad 12, member of 'highly oncogenic' group (A) of human adenoviruses. Our results indicate that the length of the inverted terminal repetition of Ad 7 (Greider) is identical to Ad 3 but different from Ad 2 (or Ad 5) and Ad 12 (or Ad 18). The nucleotide sequence homology in this unique region between Ad 7 and Ad 3 is 95%. We purified the Ad 7 DNA-protein complex to establish conditions for DNA-transfection in Human Embryonic Kidney cells in vitro.

• Research Organization: Maryland Univ., Baltimore (USA). Dept. of Biological Chemistry
• OSTI ID: 6886885
• Report Number(s): AD-A-139668/8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
DNA SEQUENCING
GENES
GENETIC MAPPING
BIOASSAY
CELL CULTURES
CHROMOSOMES
ENDONUCLEASES
MAN
ONCOGENIC TRANSFORMATIONS
PROTEINS
ANIMALS
DNA-ASE
ENZYMES
ESTERASES
HYDROLASES
MAMMALS
MAPPING
MICROORGANISMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550200* - Biochemistry