Human lymphocyte-specific pp52 gene is a member of a highly conserved dispersed family
- Univ. of California, Los Angeles (United States)
For a better understanding of genes that potentially function in B lymphocyte cell signaling, the authors isolated the human genomic counterpart of the murine pp52 or LSP1 gene. They unexpectedly found that the human pp52 gene is one of four closely related loci. Representative cosmids from each of the four family members were isolated and chromosomally localized by fluorescence in situ hybridization. Nucleotide sequence was obtained from an exon common to each locus and demonstrated very close similarity among all four loci. Two of the four loci harbored dysfunctional frameshift mutations or premature translation stop sites. The exon of one locus was flanked by an 80-bp perfect inverted repeat, suggesting that it may have originated through a looped intermediate DNA structure. Through a series of cDNA hybridization studies and nucleotide sequence analyses the authors were able to unambiguously link the lymphocyte-expressed gene to the locus mapped to chromosome 11p15.5. This same chromosomal band has been involved in tumor-related chromosomal translocations found in chronic lymphocytic leukemia. 21 refs., 4 figs.
- OSTI ID:
- 6886334
- Journal Information:
- Genomics; (United States), Vol. 15:3; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HUMAN CHROMOSOMES
GENETIC MAPPING
LYMPHOCYTES
GENES
COSMIDS
DNA
FLUORESCENCE
HYBRIDIZATION
LEUKEMIA
MUTATIONS
TRANSLOCATION
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DISEASES
IMMUNE SYSTEM DISEASES
LEUKOCYTES
LUMINESCENCE
MAPPING
MATERIALS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SOMATIC CELLS
550400* - Genetics