An experimentally derived data set constructed for testing large-scale DNA sequence assembly algorithms

Journal Article · Mon Mar 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1123· OSTI ID:6886127

Seto, D; Koop, B F; Hood, L ^[1]

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1. California Inst. of Technology, Pasadena (United States)

A data set consisting of DNA sequences from a large-scale shotgun DNA cloning and sequencing project has been collected and posted for public release. The purpose is to propose a standard genomic DNA sequencing data set by which various algorithms and implementations can be tested. This set of data is divided into two subsets, one containing raw DNA sequence data (1023 clones) and the other consisting of the corresponding partially refined or edited DNA sequence data (820 clones). Suggested criteria or guidelines for this data refinement are presented so that algorithms for preprocessing and screening raw sequences may be developed. Development of such preprocessing, screening, aligning, and assembling algorithms will expedite large-scale DNA sequencing projects so that the complete unambiguous consensus DNA sequences will be made available to the general research community in a quicker manner. Smaller scale routine DNA sequencing projects will also be greatly aided by such computational efforts. 8 refs., 2 tabs.

• DOE Contract Number: FG03-91ER61182
• OSTI ID: 6886127
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:3; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALGORITHMS
CLONING
DATA
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
HYBRIDIZATION
INFORMATION
MATHEMATICAL LOGIC
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SCREENING
STANDARDS
STRUCTURAL CHEMICAL ANALYSIS
TESTING