Anesthetic metabolism: toxic effects in or personnel. Progress report, 1 June 1977-20 December 1980. [Halothane]
Studies were conducted on the binding of halothane metabolites to human liver and gonads. Possible metabolism of nitrous oxide was also studied using isotope ratio mass spectrometry and a method of electron paramagnetic resonance spin trapping. A gas chromatograph/mass spectrometry technique was developed to identify and quantify the volatile metabolites of halothane produced in patients and in-vitro incubations. The most toxic metabolite was formed in the patient's breathing circuit, mainly from contact of halothane with hot, wet soda lime. Toxicity and mutagenicity studies indicated that some of the reductive metabolites were moderately mutagenic. Efforts were undertaken to develop a synthetic system in which drug metabolism can be studied using highly purified single forms of cytochrome-P-450 proteins in a membrane environment of exactly defined composition and physical properties. An aim was to ultimately develop a system in which drug toxicity could be tested in human liver cell components without exposing humans to harmful drugs.
- Research Organization:
- Stanford Univ., CA (USA)
- OSTI ID:
- 6881328
- Report Number(s):
- PB-88-247945/XAB
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANESTHETICS
HALOGENATED ALIPHATIC HYDROCARBONS
NITROUS OXIDE
INDOOR AIR POLLUTION
HAZARDOUS MATERIALS
GONADS
LABORATORY ANIMALS
LIVER
MEMBRANES
METABOLISM
MUTAGENS
PHOSPHOLIPIDS
PROGRESS REPORT
TOXICITY
AIR POLLUTION
BODY
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHALCOGENIDES
DIGESTIVE SYSTEM
DOCUMENT TYPES
DRUGS
ESTERS
GLANDS
LIPIDS
MATERIALS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
POLLUTION
500200* - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)
560300 - Chemicals Metabolism & Toxicology