Use of plant cell cultures to study the metabolism of environmental chemicals

Journal Article · Sun Apr 01 04:00:00 EST 1984 · Ecotoxicol. Environ. Saf.; (United States)

DOI:https://doi.org/10.1016/0147-6513(84)90059-9· OSTI ID:6880092

Sandermann, H Jr; Scheel, D; v d Trenck, T

The metabolism of the following environmental chemicals has been studied in cell suspension cultures of wheat (Triticum aestivum L.) and soybean (Glycine max L.):2, 4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), hexachlorobenzene, pentachlorophenol, diethylhexylphthalate , benzo (alpha) pyrene, and DDT. All chemicals tested, including the persistent ones, were partially metabolized. Polar conjugates predominated in all cases. A covalent incorporation into lignin could be demonstrated for 2,4-D and pentachlorophenol. A specific deposition in the cellular vacuole could be demonstrated for the beta-D-glucopyranoside conjugates derived from 2,4-D. A rapid assay procedure to evaluate the metabolism of a given /sup 14/C-labeled chemical in plant cell suspension cultures is described. This procedure requires about 1 week, and the reproducibility of the results obtained has been assessed.

• Research Organization: Institut fuer Biologie II, Universitaet Freiburg, Federal Republic of Germany
• OSTI ID: 6880092
• Journal Information: Ecotoxicol. Environ. Saf.; (United States), Journal Name: Ecotoxicol. Environ. Saf.; (United States) Vol. 8:2; ISSN EESAD
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BENZOPYRENE
BIOASSAY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CULTURES
CEREALS
CHLORINATED AROMATIC HYDROCARBONS
CONDENSED AROMATICS
DDT
GLYCINE HISPIDA
GRAMINEAE
GRASS
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
HYDROXY COMPOUNDS
INSECTICIDES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
LIGNIN
METABOLISM
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PESTICIDES
PHENOLS
PLANTS
POLYSACCHARIDES
SACCHARIDES
TRACER TECHNIQUES
WHEAT