Fluorescence image of a single molecule in a microsphere: model
- Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783 (United States)
- TRW Inc., One Space Park Drive, Redondo Beach, California 90278 (United States)
- Microparticle Photophysics Laboratory, Polytechnic University, Brooklyn, New York 11021 (United States)
- Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6142 (United States)
We model fluorescence images of single molecules in spherical dielectric microcavities. Molecules are treated as time-harmonic dipoles. Images are integrated over emission frequencies. Because of the strong refractive properties of the enclosing sphere, the fluorescence image depends on the refractive index of the sphere and the position, the orientation, and the emission frequency of the molecule. When the dipole{close_quote}s emission is at the frequency of a microsphere resonance, the brightest regions in the images appear to originate from outside the sphere for some dipole positions. This type of calculation should help in interpreting images of molecules in microspheres. {copyright} 1999 Optical Society of America
- OSTI ID:
- 688007
- Journal Information:
- Journal of the Optical Society of America, Part B: Optical Physics, Journal Name: Journal of the Optical Society of America, Part B: Optical Physics Journal Issue: 11 Vol. 16; ISSN JOBPDE; ISSN 0740-3224
- Country of Publication:
- United States
- Language:
- English
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