Urease testing of mycobacteria with BACTEC radiometric instrumentation
A total of 140 mycobacterial isolates from patients treated at Fitzsimons Army Medical Center or the National Jewish Hospital and Research Center and from animal specimens submitted to the National Veterinary Services Laboratory were tested by using a urease procedure modified for use with a BACTEC model 301. Mycobacterial suspensions were prepared by using Middlebrook 7H10 Tween broth. Of the 98 mycobacteria isolates which were urease positive utilizing standard methodology, all were positive using the radiometric procedures. Similarly, all 42 urease-negative isolates were also negative employing the new methodology. Although maximum radiometric readings were observed at 48 h, all positive strains were readily identified 24 h after inoculation without sacrificing either test sensitivity or specificity. Thus, urease testing of mycobacteria, using the modified BACTEC radiometric methodology, was as sensitive, as specific, and more rapid than conventional methods.
- Research Organization:
- Clinical Investigation Service, Fitzsimons Army Medical Center, Aurora, Colorado
- OSTI ID:
- 6879656
- Journal Information:
- J. Clin. Microbiol.; (United States), Journal Name: J. Clin. Microbiol.; (United States) Vol. 15:3; ISSN JCMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMIDINASES
BACTERIA
BACTERIAL DISEASES
CARBON ISOTOPES
CHEMICAL ANALYSIS
DIAGNOSIS
DISEASES
ENZYMES
HYDROLASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
MICROORGANISMS
MYCOBACTERIUM
NON-PEPTIDE C-N HYDROLASES
PATIENTS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOMETRIC ANALYSIS
UREASE