Basolateral K+ channels in airway epithelia. II. Role in Cl- secretion and evidence for two types of K+ channel

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · American Journal of Physiology; (USA)

OSTI ID:6874813

McCann, J D; Welsh, M J ^[1]

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1. Univ. of Iowa College of Medicine, Iowa City (USA)

We previously described a Ca2(+)-activated K+ channel (KCLIC) in airway epithelial cells. To determine whether the KCLIC channel is a basolateral membrane channel and to understand its role in Cl- secretion, we studied airway epithelial cells grown on permeable supports. When cells were stimulated with A23187, charybdotoxin (ChTX) inhibited Cl- secretion and 86Rb efflux at the same concentrations, indicating that the KCLIC channel is required for Ca2(+)-stimulated Cl- secretion. We also investigated the function of K+ channels in adenosine 3',5'-cyclic monophosphate-stimulated secretion. Addition of isoproterenol caused a biphasic increase in Cl- secretion; the time course of the transient component correlated with the time course of the isoproterenol-induced increase in Ca2+ concentration (( Ca2+)c). ChTX inhibited the transient component, but not the prolonged component of secretion; Ba2+ inhibited the sustained component. These results suggest that when cells are grown on permeable supports isoproterenol-induced secretion depends on activation of two types of K+ channel: the KCLIC channel that is stimulated initially and a ChTX-insensitive K+ channel that is stimulated during sustained secretion. This conclusion was supported by measurement of 86Rb efflux from cell monolayers.

• OSTI ID: 6874813
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 258; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALINE EARTH METALS
AMP
ANIMAL TISSUES
ANIMALS
ANTIGENS
BARIUM
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DOGS
ELECTROPHYSIOLOGY
ELEMENTS
EPITHELIUM
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
MEMBRANES
METALS
MINUTES LIVING RADIOISOTOPES
MUSCLES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHYSIOLOGY
POTASSIUM COMPOUNDS
RADIOISOTOPES
RESPIRATORY SYSTEM
RUBIDIUM 86
RUBIDIUM ISOTOPES
SECRETION
TISSUES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRACHEA
VERTEBRATES