Monitoring of aflatoxins and ochratoxin A in Czechoslovak human sera by immunoassay

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Bulletin of Environmental Contamination and Toxicology; (USA)

DOI:https://doi.org/10.1007/BF01701213· OSTI ID:6870067

Fukal, L ^[1]; Reisnerova, H ^[2]

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1. Institute of Chemical Technology, Prague (Czechoslovakia)
2. Univ. of Agriculture, Prague (Czechoslovakia)

Since a level of food contamination with aflatoxins and ochratoxin A has been found low in Czechoslovakia, human exposure to these mycotoxins may not be negligible. However, analysis of food samples provides only indirect evidence of mycotoxin ingestion and no evidence about mycotoxin absorption. Direct evidence can only be obtained by analysis of human body fluids. Therefore, the authors decided to carry out a monitoring of aflatoxin and ochratoxin A level in human sera. In general, TLC and HPLC are most commonly used to analyze mycotoxins and its metabolites. The recent development of immunochemical techniques opens the possibility of determining individual exposure in a relatively large human population. These assays have the advantage of high specificity and sensitivity. Sample through-put is high, and the methods are technically simple and can be performed at low cost.

• OSTI ID: 6870067
• Journal Information: Bulletin of Environmental Contamination and Toxicology; (USA), Journal Name: Bulletin of Environmental Contamination and Toxicology; (USA) Vol. 44:3; ISSN 0007-4861; ISSN BECTA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AFLATOXIN
AMINO ACIDS
ANIMALS
ANTIGENS
BIOASSAY
BLOOD SERUM
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CZECHOSLOVAKIA
DEVELOPING COUNTRIES
DIAGNOSTIC TECHNIQUES
EASTERN EUROPE
EUROPE
FUNGI
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIGANDS
MAMMALS
MAN
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENYLALANINE
PLANTS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VERTEBRATES