One- and two-dimensional NMR studies on the conformation of DNA containing the oligo(dA)oligo(dT) tract
- Osaka Univ. (Japan)
The resonances of the imino protons and all of the non-exchangeable protons (except for H5{prime}/H5{double prime}) of d(CGCAAAAAACGC)d(CGCTTTTTTGCG) have been assigned by means of one- and two-dimensional NMR spectroscopies. Qualitative analyses showed that the overall structure is of the B-form, but local conformational deviations exist. The NOEs between the imino protons of thymines and H2 of adenines suggest that the A-T base pairs are propeller-twisted to almost the same degree as in crystals. A remarkable chemical shift of H1{prime} was observed for the residue located just before the oligo(dA)oligo(dT) tract, suggesting the presence of conformational discontinuity at the junctions between the oligo(dA)oligo(dT) tract and the other portions. Analyses of cross peaks in NOESY spectra between H2 of adenines and H1{prime} of the 3{prime}-neighboring residues on the complementary strand revealed that the minor groove of the oligo(dA)oligo(dT) tract is narrow and compressed gradually from 5{prime} to 3{prime}, along the tract.
- OSTI ID:
- 6870060
- Journal Information:
- Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:17; ISSN 0305-1048; ISSN NARHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Structural analysis of d(GCAATTGC) sub 2 and its complex with berenil by nuclear magnetic resonance spectroscopy
Poly(dA-dT). poly(dA-dT) two-pathway proton exchange mechanism. Effect of general and specific base catalysis on deuteration rates
Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
AZINES
BARYONS
CONFORMATIONAL CHANGES
DNA
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMINES
MAGNETIC RESONANCE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVERHAUSER EFFECT
PROTONS
PYRIMIDINES
RESONANCE
THYMINE
URACILS