Radiosensitivity of fibroblasts obtained from a cafe-au-lait spot and normal-appearing skin of a patient with neurofibromatosis (NF-6)

Journal Article · Sun Jul 15 00:00:00 EDT 1990 · Cancer Genetics and Cytogenetics; (USA)

DOI:https://doi.org/10.1016/0165-4608(90)90029-A· OSTI ID:6869884

Hannan, M A; Smith, B P; Sigut, D; Sackey, K ^[1]

---

1. King Faisal Specialist Hospital and Research Centre, Riyadh (Saudi Arabia)

Fibroblast cells derived from a cafe-au-lait spot and normal-appearing skin of a neurofibromatosis (NF-6) patient were studied for radiosensitivity in comparison with two normal cell lines used as controls. No difference in radiosensitivity was observed between the patient's cell lines and the controls using acute gamma-irradiation. However, a markedly increased radiosensitivity of the fibroblasts obtained from the patient's skin of normal appearance was demonstrated after chronic gamma-irradiation. The cells from the cafe-au-lait spot showed intermediate sensitivity to chronic irradiation as compared with the control cell lines and the fibroblasts derived from the normal skin of the patient. These results showed the usefulness of chronic irradiation in detecting increased cellular radiosensitivity which may result from a unique DNA repair defect in an NF patient. We suggest that enhanced genetic changes in radiosensitive NF patients may lead to formation of cafe-au-lait lesions and certain tumors. Such a transformation may be associated with production of radiotolerant cells.

• OSTI ID: 6869884
• Journal Information: Cancer Genetics and Cytogenetics; (USA), Vol. 47:2; ISSN 0165-4608
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
FIBROBLASTS
RADIOSENSITIVITY
SKIN
NEOPLASMS
ACUTE IRRADIATION
CHRONIC IRRADIATION
DNA REPAIR
GAMMA RADIATION
MAN
PATIENTS
RADIATION DOSES
SURVIVAL TIME
TUMOR CELLS
ACUTE EXPOSURE
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CHRONIC EXPOSURE
CONNECTIVE TISSUE CELLS
DISEASES
DOSES
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
ORGANS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture