Studies on spin-trapped radicals in. gamma. -irradiated aqueous solutions of glycine and L-alanine by high-performance liquid chromatography and ESR spectroscopy

Journal Article · Wed Nov 12 23:00:00 EST 1980 · J. Phys. Chem.; (United States)

DOI:https://doi.org/10.1021/j100460a023· OSTI ID:6869029

Moriya, F; Makino, K; Suzuki, N; Rokushika, S; Hatano, H

Aqueous solutions of glycine and L-alanine were ..gamma.. irradiated in the presence of a spin trap, 2-methyl-2-nitrosopropane. Stable spin adducts produced in the ..gamma..-irradiated solutions were analyzed by means of high-performance liquid chromatography and ESR spectroscopy. The following four spin adducts were found and identified: t-BuN(O.)CH/sub 2/COO/sup -/(I) and t-BuN(O.)CH(NH/sub 2/)COO/sup -/(II) from glycine; t-BuN(O.)CH(CH/sub 3/)COO/sup -/(III) and t-BuN(O.)CH/sub 2/CH(NH/sub 3//sup +/)COO/sup -/(IV) from L-alanine. It was found that the ESR spectra of these spin adducts changed with pH through the acid dissociation equilibria of the carboxyl or amino groups. The pK/sub a/ values for the dissociation have been determined to be 3.2 for the carboxyl group of spin adduct III, and 8.9 for the amino group of IV.

• Research Organization: Kyoto Univ., Japan
• OSTI ID: 6869029
• Journal Information: J. Phys. Chem.; (United States), Journal Name: J. Phys. Chem.; (United States) Vol. 84:23; ISSN JPCHA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400600* -- Radiation Chemistry
ALANINE-ALPHA
ALANINE-L
ALANINES
AMINO ACIDS
AQUEOUS SOLUTIONS
CARBOXYLIC ACIDS
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CHROMATOGRAPHY
DATA
DISPERSIONS
ELECTROMAGNETIC RADIATION
ELECTRON SPIN RESONANCE
EXPERIMENTAL DATA
GAMMA RADIATION
GLYCINE
INFORMATION
IONIZING RADIATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAGNETIC RESONANCE
MIXTURES
NITROSO COMPOUNDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADICALS
RESONANCE
SCAVENGING
SEPARATION PROCESSES
SOLUTIONS
SPECTRA