Interaction of human plasma high density lipoprotein HDL/sub 2/ with synthetic saturated phosphatidylcholines
The interaction of human plasma high density lipoprotein HDL/sub 2/ (d 1.063 to 1.125 g/ml) with sonicated dispersions of synthetic saturated phosphatidylcholines, dipalmitoyl- (diC/sub 16/PC), dimyristoyl-(diC/sub 14/PC), didodecanoyl-(diC/sub 12/PC), didecanoyl-(diC/sub 10/PC), and dioctanoyl-(diC/sub 8/PC) L-alpha phosphatidylcholine, was investigated. Incubation (4.5 hr, 37 C) of HDL/sub 2/ with diC/sub 14/PC, diC/sub 12/PC, diC/sub 10/PC and diC/sub 8/PC followed by gradient gel electrophoresis or preparative ultracentrifugation resulted in a redistribution of apolipoprotein A-I (apoA-I). The extent of redistribution depended on the molar ratio of the phospholipid to HDL/sub 2/ in the incubation mixture. Redistributed apoA-I occurred as lipid-free apoA-I and/or as complexes of apoA-I with phosphatidylcholine. Increasing the length of time of ultracentrifugation of the interaction mixtures did not increase the extent of redistribution. No redistribution of apoA-I was detected following incubation and gradient gel electrophoresis or preparative ultracentrifugation of mixtures of HDL/sub 2/ with dispersions of diC/sub 16/PC.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 6868926
- Journal Information:
- Lipids; (United States), Vol. 15:2
- Country of Publication:
- United States
- Language:
- English
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550200* - Biochemistry