Reevaluation of interaction of cis-dichloro(ethylenediamine)platinum(II) with DNA

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00361a026· OSTI ID:6868284

Eastman, A

Intrastrand cross-links represent the majority of modifications in DNA resulting from interaction with the cancer chemotherapeutic drug cis-diamminedichloroplatinum(II) (cis-DDP). These adducts were recently characterized although several discrepancies remained to be resolved. In these studies, (/sup 3/H)-cis-dichloro(ethylenediamine)platinum(II) (cis-DEP) was used because of the convenience of the radiolabel; this analogue produces adducts at identical sites in DNA at cis-DDP. The adduct at AG sequences invariably has adenine on the 5'-terminus of the dimer. After short incubations, with cis-DEP and removal of unreacted drug, the monofunctional adducts slowly rearranged to bifunctional adducts. It is suggested that this evolution of adducts may result from the drug walking along the double helix, a phenomenon that does not appear to occur in single-stranded DNA.

• Research Organization: Univ. of Nebraska Medical Center, Omaha (USA)
• OSTI ID: 6868284
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 25:13; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMOTHERAPY
DISEASES
DNA
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PLATINUM COMPOUNDS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
THERAPY
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
TRITIUM
YEARS LIVING RADIOISOTOPES