Normal mitogen-induced suppression of the interleukin-6 (IL-6) response and its deficiency in systemic lupus erythematosus

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Journal of Clinical Immunology; (USA)

DOI:https://doi.org/10.1007/BF00917498· OSTI ID:6868261

Warrington, R J; Rutherford, W J ^[1]

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1. Univ. of Manitoba, Winnipeg (Canada)

A low-frequency suppressor-cell population in normal peripheral blood inhibits the B-cell CESS response to IL-6, following pokeweed mitogen stimulation. The suppression of IL-6 responsiveness is radiation sensitive, directed against CESS targets and not mediated by inhibition of IL-6 production, and associated with nonspecific cytotoxic activity against CESS targets. The generation of these cytolytic cells is also radiation sensitive. A correlation was found between PWM-induced cytotoxicity against CESS and the suppression of IL-6-dependent IgG production. But cytotoxicity toward CESS targets is not responsible for this suppression because IL-2 induces equivalent or greater nonspecific cytotoxicity against CESS in the total absence of suppression of CESS-derived IgG production and suppression is also induced by mitogen-activated PBL separated from CESS targets by a cell-impermeable membrane. This suppression was not mediated by TNF alpha/beta or IFN-gamma. In systemic lupus erythematosus, suppression of IL-6-dependent IgG production is impaired in patients with active disease (29.2 +/- 13.7%) compared to patients with inactive disease (70 +/- 19.5%) or normal controls (82.8 +/- 9.2%). There is also a defect in mitogen-induced nonspecific cytotoxicity in active SLE (specific lysis 15.1 +/- 3.5%, compared to 34 +/- 4% in normals). Pokeweed mitogen-activated PBL can therefore normally induce suppression of B-cell IL-6 responses and this response is deficient in lupus.

• OSTI ID: 6868261
• Journal Information: Journal of Clinical Immunology; (USA), Vol. 10:1; ISSN 0271-9142
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
IMMUNE SYSTEM DISEASES
PATHOGENESIS
LYMPHOCYTES
RADIOSENSITIVITY
LYMPHOKINES
IMMUNOSUPPRESSION
BIOSYNTHESIS
CELL MEMBRANES
IMMUNOGLOBULINS
INHIBITION
MAN
MITOGENS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DISEASES
GLOBULINS
GROWTH FACTORS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANES
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550600 - Medicine
550900 - Pathology