The N2-guanine adduct but not the C8-guanine or N6-adenine adducts formed by 4-nitroquinoline 1-oxide blocks the 3'-5' exonuclease action of T4 DNA polymerase
- Univ. of Western Ontario, London (Canada)
When O-acetyl-4-(hydroxyamino)quinoline 1-oxide (Ac-4HAQO) reacts with double-stranded DNA at 37 degrees C the major products, N2-guanine, C8-guanine, and N6-adenine adducts, are formed in the proportions of 5:3:2, respectively. When the reaction is carried out with single-stranded DNA at 0 degree C, the products are found in the ratio 1:7:2. Unique 174-bp DNA fragments were modified in these ways and used as substrates for the 3'-5' exonuclease activity of T4 DNA polymerase. The results obtained showed that the exonuclease is blocked by the N2-guanine adduct but not the other two adducts. Interpretation of the cleavage patterns suggested that the enzyme stopped 2 nucleotides before the N2-guanine adduct. The N2-guanine adduct lies in the minor groove of the DNA double helix, while the other two adducts are found in the major groove. Apparently, only the former hinders progression of the enzyme.
- OSTI ID:
- 6867171
- Journal Information:
- Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:8; ISSN 0006-2960; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ADENINES
AMINES
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
AZINES
BACTERIOPHAGES
BIOSYNTHESIS
CATTLE
DNA
DNA ADDUCTS
DNA POLYMERASES
DOMESTIC ANIMALS
DRUGS
ENZYME ACTIVITY
ENZYMES
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INHIBITION
MAMMALS
METABOLISM
MICROORGANISMS
NITRO COMPOUNDS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PURINES
PYRIDINES
QUINOLINES
RUMINANTS
SYNTHESIS
TRANSFERASES
VERTEBRATES
VIRUSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ADENINES
AMINES
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
AZINES
BACTERIOPHAGES
BIOSYNTHESIS
CATTLE
DNA
DNA ADDUCTS
DNA POLYMERASES
DOMESTIC ANIMALS
DRUGS
ENZYME ACTIVITY
ENZYMES
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INHIBITION
MAMMALS
METABOLISM
MICROORGANISMS
NITRO COMPOUNDS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PURINES
PYRIDINES
QUINOLINES
RUMINANTS
SYNTHESIS
TRANSFERASES
VERTEBRATES
VIRUSES