The kinetic study of hydrogen bacteria and methanotrophs in pure and defined mixed cultures
The kinetics of pure and mixed cultures of Alcaligenes eutrophus H 16 and Methylobacterium organophilum CRL 26 under double substrate limited conditions were studied. In pure culture growth kinetics, a non-interactive model was found to fit the experimental data best. The yield of biomass on limiting substrate was found to vary with the dilution rate. The variation in the biomass yield may be attributed to the change in metabolic pathways resulting from a shift in the limiting substrates. Both species exhibited wall growth in the chemostat under dark conditions. However, under illuminated conditions, there was significant reduction in wall growth. Poly-{beta}-hydroxybutyric acid was synthesized by both species under ammonia and oxygen limiting conditions. The feed gas mixture was optimized to achieve the steady-state coexistence of these two species in a chemostate for the first time. In mixed cultures, the biomass species assays were differentiated on the basis of their selective growth on particular compounds: Sarcosine and D-arabinose were selected for hydrogen bacteria and methylotrophs, respectively. The kinetics parameters estimated from pure cultures were used to predict the growth kinetics of these species in defined mixed cultures.
- Research Organization:
- California Univ., Davis, CA (USA)
- OSTI ID:
- 6865468
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
08 HYDROGEN
BACTERIA
GROWTH
BIOMASS
BUTYRIC ACID
HYDROGEN PRODUCTION
METABOLISM
METABOLITES
METHANOGENIC BACTERIA
SUBSTRATES
CARBOXYLIC ACIDS
ENERGY SOURCES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RENEWABLE ENERGY SOURCES
550700* - Microbiology
560300 - Chemicals Metabolism & Toxicology
080100 - Hydrogen- Production