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Title: Continuous-sterilization system that uses photosemiconductor powders. [Escherichia coli]

Journal Article · · Appl. Environ. Microbiol.; (United States)
OSTI ID:6861269

We report a novel photochemical sterilization system in which Escherichia coli cells were sterilized with photosemiconductor powders (titanium oxide). For sterilization that could be used in practice, it was necessary to separate the TiO/sub 2/ powders from the cell suspension. Therefore, semiconductor powders were immobilized on acetylcellulose membranes. We constructed a continuous-sterilization system consisting of TiO/sub 2/-immobilized acetylcellulose membrane reactor, a mercury lamp, and a masterflex pump. As a result, under the various sterilization conditions examined, E.coli (10/sup 2/ cells per ml) was sterilized to < 1% survival when the cell suspension flowed in this system at a mean residence time of 16.0 min under irradiation (1800 microeinsteins/m/sup 2/ per s). We found that this system was reusable.

Research Organization:
Tokyo Univ. of Agriculture and Technology (Japan)
OSTI ID:
6861269
Journal Information:
Appl. Environ. Microbiol.; (United States), Vol. 54:6
Country of Publication:
United States
Language:
English