High pressure liquid chromatographic method for the separation and quantitation of water-soluble radiolabeled benzene metabolites
The glucuronide and sulfate conjugates of benzene metabolite as well as muconic acid and pre-phenyl- and phenylmercapturic acids were separated by ion-pairing HPLC. The HPLC method developed was suitable for automated analysis of a large number of tissue or excreta samples. p-Nitrophenyl (/sup 14/C)glucuronide was used as an internal standard for quantitation of these water-soluble metabolites. Quantitation was verified by spiking liver tissue with various amounts of phenylsulfate or glucuronides of phenol, catechol, or hydroquinone and analyzing by HPLC. Values determined by HPLC analysis were within 10% of the actual amount with which the liver was spiked. The amount of metabolite present in urine following exposure to (/sup 3/H)benzene was determined using p-nitrophenyl (/sup 14/C)glucuronide as an internal standard. Phenylsulfate was the major water-soluble metabolite in the urine of F344 rats exposed to 50 ppm (/sup 3/H)benzene for 6 h. Muconic acid and an unknown metabolite which decomposed in acidic media to phenylmercapturic acid were also present. Liver, however, contained a different metabolic profile. This indicates that urinary metabolite profiles may not be a true reflection of what is seen in individual tissues.
- Research Organization:
- Lovelace Inhalation Toxicology Research Institute, Albuquerque, NM (USA)
- DOE Contract Number:
- AC04-76EV01013
- OSTI ID:
- 6861209
- Journal Information:
- Anal. Biochem.; (United States), Vol. 170:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
BENZENE
METABOLISM
BIOLOGICAL MATERIALS
QUANTITATIVE CHEMICAL ANALYSIS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
CARBON 14 COMPOUNDS
FECES
GLUCURONIDE CONJUGATES
LIVER
TRITIUM COMPOUNDS
URINE
AROMATICS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
GLANDS
HYDROCARBONS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
SEPARATION PROCESSES
WASTES
560300* - Chemicals Metabolism & Toxicology
550601 - Medicine- Unsealed Radionuclides in Diagnostics