Conjugative transfer of cadmium resistance plasmids in Rhodococcus fascians strains

Journal Article · Sun May 01 00:00:00 EDT 1988 · J. Bacteriol.; (United States)

OSTI ID:6861168

Desomer, J; Dhaese, P; Montagu, M V

The presence of a 138-kilobase plasmid (pD188) correlated with increased resistance to cadmium in Rhodococcus fascians D188. This plasmid could be transferred by a conjugation-like system in matings between R. fascians strains. To examine this correlation we used large /sup 32/P-labeled pD188 subclones as probes in hybridization analyses with Southern blots of restricted total DNAs of D188 and its derivative mutants. Transconjugants expressed the cadmium resistance and could be used as donors in subsequent matings. Four other R. fascians strains (NCPPB 1488, NCPPB 1675, NCPPB 2551, and ATCC 12974) could also be used as donors for cadmium resistance in matings. Strain NCPPB 1675 showed a 100% cotransfer of cadmium and chloramphenicol resistance markers.

• Research Organization: Rijksuniversiteit Gent (Belgium)
• OSTI ID: 6861168
• Journal Information: J. Bacteriol.; (United States), Vol. 170:5
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CADMIUM
BIOLOGICAL EFFECTS
PATHOGENS
GENE REGULATION
SENSITIVITY
DNA
HYBRIDIZATION
PHOSPHORUS 32
PLASMIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ISOTOPES
LIGHT NUCLEI
METALS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
560300* - Chemicals Metabolism & Toxicology
550400 - Genetics