Molecular cloning, sequencing, and characterization of smooth muscle myosin alkali light chain from human eye cDNA: Homology with myocardial fatty acid ethyl ester synthase-III cDNA
Journal Article
·
· Genomics; (United States)
- Washington Univ. School of Medicine, St. Louis, MO (United States)
- CV Therapeutics, Inc., Mountain View, CA (United States)
As an approach to obtaining more information on the structure and function of the detoxification enzyme glutathione S-transferase (GST) from human eye, the authors screened a human eye [lambda]gt11 cDNA library with fatty acid ethyl ester synthase-III (FAEES-III) cDNA. This cDNA encodes an acidic isoenzyme of the GSTs that metabolizes ethanol and can be classified as a [pi] GST. Sequence analysis of a selected clone indicated it to be a smooth muscle myosin alkali light chain, with the nucleotide sequence having 54% homology with the human myocardial FAEES-III nucleotide sequence. PCR analysis demonstrated the expression of ESMLC in aorta, eye, and heart. 10 refs., 2 figs.
- OSTI ID:
- 6860488
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:1; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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