Regulation of IL-2 induced proliferation and cytotoxicity in human natural killer cells by monoclonal antibodies
Natural killer (NK) activity is mediated by a subpopulation of cells termed large granular lymphocytes (LGL), which exhibit cytotoxic activity against a variety of tumor targets. LGL express OKT8, OKT9, OKT10, OKT11, 3G8 (Fc..gamma..R), OKM1, NKH1. The addition of recombinant IL-2 (rIL-2), increases cytotoxicity, induces IFN-..gamma.. production and leads to LGL proliferation. Since monoclonal antibodies (MoAb) represent highly specific probes to analyze possible surface molecules, they have studied the role of various MoAbs in the regulation of cytotoxicity, proliferation, and secretory function of purified LGL. LGL were isolated from nonadherent human peripheral blood leukocytes on discontinuous Percoll density gradients, followed by 29/sup 0/C E-rosette depletion of contaminating T cells. These preparations were greater than or equal to 85% LGL and contained greater than or equal to 5% OKT3/sup +/ cells. Using a limiting dilution assay, purified LGL were incubated with rIL-2 and the MoAbs (10 ..mu..g/ml) for 7 days. These cells were tested for cytotoxicity against K562 in a /sup 51/Cr/sup -/ release assay, and for proliferation as determined by /sup 3/H-thymidine incorporation. Results indicate that the OKT9 antibody inhibited both the cytotoxicity and proliferation. MoAb against LGl markers (OKT11, OKT8, OKM1, 3G8, and NKH1) had no effect on cytotoxicity or proliferation. Unlike the T cell receptor complex (with OKT3), the surface molecules examined do not regulate LGL lysis or proliferation.
- OSTI ID:
- 6859615
- Report Number(s):
- CONF-8604222-; TRN: 87-010329
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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