Adenylate cyclase activity, (/sup 3/H)-dihydroalprenolol binding and fatty acid composition of rat heart membranes in essential fatty acid deficiency
Three groups of male, weanling, Sprague-Dawley rats were fed essential fatty acid (EFA) deficient (EFAD), marginally EFA-deficient (MEFAD) or control diets up to 17 weeks. Plasma membranes from heart of EFAD and MEFAD rats had lower basal and fluoride-stimulated adenylate cyclase activity as compared with the controls. The double bond index of total lipids and phospholipids, and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene were not appreciably different in the membranes from the 3 dietary groups. The fatty acid composition of total lipids and phospholipids showed changes typical of an EFA deficiency. The feeding of control diet to the EFAD rats for 6 weeks did not alleviate completely the changes in adenylate cyclase activity although the fatty acid patterns were restored to the normal levels. There was also a decrease in the number of ..beta.. -adrenergic receptors in heart of EFAD rats as compared with controls. The results suggest that, in addition to membrane fluidity, changes in the number of ..beta.. -adrenergic receptors and other metabolic changes induced by EFA deficiency may be important in regulating adenylate cyclase activity in heart.
- Research Organization:
- Louisiana State Univ. Medical Center, New Orleans
- OSTI ID:
- 6859350
- Report Number(s):
- CONF-8604222-; TRN: 87-010349
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXYLIC ACIDS
NUTRITIONAL DEFICIENCY
CELL MEMBRANES
CHEMICAL COMPOSITION
CYCLASES
ENZYME ACTIVITY
SYMPATHOLYTICS
BIOCHEMICAL REACTION KINETICS
HEART
LIPIDS
PHOSPHOLIPIDS
RATS
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
DRUGS
ENZYMES
ESTERS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYASES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques