New metabolites of vitamin B/sub 6/ in cat urine
Journal Article
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6858337
Plasma from adult cats and from humans with adult onset hypophosphatasia has low alkaline phosphatase activity and high pyridoxal phosphate (PLP). Since the cats do not show the bone disease seen in humans, the authors initiated a study of vitamin B/sub 6/ metabolism in cats. /sup 14/C-pyridoxine HCL was donated by Hoffmann LaRoche (Nutley, NJ) and purified by HPLC. Following oral doses of /sup 14/C-pyridoxine HCL ranging from .97 to 486 ..mu..mol, urinary pyridoxic acid (PA) accounted for only 1 to 5% of the dose. Cation exchange HPLC of the urine revealed about 50% of the dose in a very fast eluting peak (X) and another 20 to 25% of the dose in a very slow eluting peak (Y). X elutes faster than PLP, is stable in base, but is hydrolyzed by acid to pyridoxal and pyridoxine. These properties are similar to those of 3-sulfate esters. Y is not altered by treatment with acid or base. In cation-exchange HPLC Y does not elute with the common B/sub 6/ vitamers but does elute with N-methyl-pyrdoxine. Y moves with N-methyl-pyridoxine in 3 TLC solvents. Both Y and N-methyl-pyridoxine fail to give an orange spot with diazotized p-nitroaniline. Further attempts to characterize X and Y are in progress. The low concentrations of PA in cat plasma and urine suggest a very limited capacity to oxidize vitamin B/sup 6/. This limitation apparently forces the use of alternate metabolic pathways.
- Research Organization:
- Ft. Wayne State Development Center, IN
- OSTI ID:
- 6858337
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBON 14 COMPOUNDS
CATS
CHROMATOGRAPHY
ENZYME ACTIVITY
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATASES
PHOSPHATES
PHOSPHORUS COMPOUNDS
PYRIDINES
PYRIDOXINE
SEPARATION PROCESSES
TRACER TECHNIQUES
URINE
VERTEBRATES
VITAMIN B GROUP
VITAMINS
WASTES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBON 14 COMPOUNDS
CATS
CHROMATOGRAPHY
ENZYME ACTIVITY
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATASES
PHOSPHATES
PHOSPHORUS COMPOUNDS
PYRIDINES
PYRIDOXINE
SEPARATION PROCESSES
TRACER TECHNIQUES
URINE
VERTEBRATES
VITAMIN B GROUP
VITAMINS
WASTES