Is glutamine oxidation necessary to support intestinal functions

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6858234

Nagy, L E; Kretchmer, N

Glutamine (GLN) is known to be a major oxidative substrate in the small intestine, but the role of GLN oxidation in the support of the critical intestinal functions of nutrient absorption and DNA synthesis is not known. In the presence and absence of GLN, rates of active transport of 3-O-methylglucose (MG) and histidine(HIS) were determined in everted sacs of rat jejunum. 10 mM MG was absorbed at equivalent rates when incubated with 10 mM GLN, 10 mM B-hydroxybutyrate (BHB) or Krebs Ringer phosphate with no added substrate. Absorption of HIS was also independent of the presence of GLN as an oxidative substrate. The dependence of DNA synthesis on GLN oxidation was determined by measuring /sup 3/H-methyl-thymidine (/sup 3/HTdR) incorporation in isolated crypt cells incubated in Media 199 which includes purines and pyrimidines to support DNA synthesis, but no GLN. The addition of 10 mM GLN to crypt cells from rats refed after a fast resulted in a 57% increase (p<0.03) in /sup 3/HTdR incorporation compared to cells with no added substrates. When either 20 mM aminooxyacetate, an inhibitor of GLN oxidation, or 0.2 mM BHB were added with the GLN, rates of /sup 3/HTdR incorporation decreased to basal values. These studies demonstrate that the jejunum can support short term active transport from endogenous fuel supplies other than GLN. However, elevated rates of /sup 3/HTdR incorporation appear to be dependent on the oxidation of GLN by jejunal crypt cells.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 6858234
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ALDEHYDES
AMIDES
AMINO ACIDS
ANIMALS
AZINES
AZOLES
BIOLOGICAL FUNCTIONS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DNA REPLICATION
FUNCTIONS
GASTROINTESTINAL TRACT
GLUCOSE
GLUTAMINE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
HISTIDINE
IMIDAZOLES
INTESTINAL ABSORPTION
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SACCHARIDES
SMALL INTESTINE
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES