Uptake and metabolism of (11-/sup 3/H) all-trans retinoic acid by rabbit tracheal epithelial cells

Journal Article · Tue Dec 31 23:00:00 EST 1985 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6857741

Bhat, P V; Jetten, A M

Retinoic acid (RA) inhibits squamous cell differentiation of rabbit tracheal epithelial cells in culture at concentrations as low as 10/sup -9/ - 10/sup -10/ M. These cells take up 11-(/sup 3/H)-RA readily when added to the cells either as free RA or as RA complexed to serum retinol binding protein (SRBP) or albumin. The uptake of RA by RTE cells as SRBP or albumin complexes was significantly lower than that of free RA. Metabolites were analyzed by high pressure liquid chromatography. This analysis showed that RTE cells metabolized RA to polar metabolites (Peak I) and to a less polar metabolite (Peak III). The metabolite in Peak III constituted 13-20% of the cell-associated radioactivity after 24 hrs. incubation with RA. Formation of the Peak I and Peak III metabolites from RA was observed both in undifferentiated as well as in cells that underwent terminal differentiation to squamous cells and their synthesis appeared constitutive. When cells were treated for 6 hrs with /sup 3/H-RA and then further in the absence of RA 75% of the cell-associated radioactivity was released in the medium within 24 hrs, thereafter the release was slow. Analysis of the metabolites secreted by the cells into the medium showed only the presence of Peak I metabolites. The authors data show that RTE cells metabolize RA into polar metabolites which are rapidly released into the medium and into a less polar metabolite, possibly an ester of retinoic acid, which is retained by the cell.

• Research Organization: Clinical Research Institute, Montreal, Quebec
• OSTI ID: 6857741
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL TISSUES
ANIMALS
BODY
CARBOXYLIC ACID ESTERS
CELL DIFFERENTIATION
CHROMATOGRAPHY
EPITHELIUM
ESTERS
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
PROTEINS
RABBITS
RESPIRATORY SYSTEM
RETINOIC ACID
SEPARATION PROCESSES
TISSUES
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES