Lumen formation by epithelial cell lines in response to collagen overlay: a morphogenetic model in culture

Journal Article · Sun Aug 01 00:00:00 EDT 1982 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.79.15.4672· OSTI ID:6857247

Hall, H G; Farson, D A; Bissel, M J

Two cells lines--Madin-Darby canine kidney (MDCK) and normal murine mammary gland (NMuMG)--growing as monolayers on collagen gels were overlaid with another collagen gel. The cells responded to the overlay by undergoing reorganization resulting in the creation of lumina. MDCK cells formed lumina that coalesced to form large cavities comparable in size with a tubule. NMuMG cells formed clusters surrounding small lumina, which appeared similar to acini of glandular tissue. The characteristic arrangements, described here by light and electron microscopy, resembled the morphology of the tissues of cell line origin. MDCK cells, grown in the presence of serum, formed lumina whether or not serum was removed at the time of overlay, whereas NMuMG cells required either a nondialyzable component of serum or hormonal supplements in serum-free defined media. Lumen formation was delayed by MDCK cells in the presence of the glutamine analog 6-diazo-5-oxo-L-norleucine, but this compound did not affect NMuMG lumen formation. In both cell lines, lumen formation was unaffected by the absence of sulfate, the presence of an inhibitor of sulfate glycosaminoglycan synthesis, or an inhibitor of collagen synthesis. DNA synthesis accompanied lumen formation but was not required.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6857247
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 79:15
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
MORPHOLOGICAL CHANGES
COLLAGEN
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CELL CULTURES
DNA
DOGS
INHIBITION
KIDNEYS
MAMMARY GLANDS
MICE
SULFATES
ANIMALS
BODY
GLANDS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PROTEINS
RODENTS
SCLEROPROTEINS
SULFUR COMPOUNDS
SYNTHESIS
VERTEBRATES
550300* - Cytology