Kinetics of leukocyte sequestration in the lungs of acutely septic primates: A study using sup 111 In-labeled autologous leukocytes

Journal Article · Thu Mar 01 00:00:00 EST 1990 · Journal of Surgical Research; (USA)

DOI:https://doi.org/10.1016/0022-4804(90)90213-L· OSTI ID:6854580

Hangen, D H; Segall, G M; Harney, E W; Stevens, J H; McDougall, I R; Raffin, T A ^[1]

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1. Stanford Univ. Medical Center, CA (USA)

To further clarify the role of leukocytes in the pathogenesis of ARDS, we studied the localization and kinetics of leukocyte migration using 111In-labeled autologous white cell scans ({sup 111}In wbc scans) in four primates made acutely septic with infusions of Escherichia coli. Whole body images were obtained with a gamma camera and were acquired on computer every 15 min beginning immediately after the E. coli infusion. Simultaneous measurements of C5a and peripheral blood leukocyte count were also obtained. Within 5 min of initiating sepsis, three major events occurred: complement activation as measured by the production of C5a, a profound fall in peripheral leukocyte count, and a significant increase in the sequestration of leukocytes in the lungs. The pulmonary sequestration reached a peak at 15 min with a mean of 152% of baseline activity. This sequestration consisted of a population that was predominantly neutrophils. Damage to the pulmonary capillary endothelium was demonstrated by an increase in extravascular lung water. The results support a role for neutrophils and complement as mediators in the pathogenesis of ARDS.

• OSTI ID: 6854580
• Journal Information: Journal of Surgical Research; (USA), Vol. 48:3; ISSN 0022-4804
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
LEUKOCYTES
RADIOISOTOPE SCANNING
RESPIRATORY SYSTEM DISEASES
DIAGNOSIS
BACTERIAL DISEASES
ENDOTHELIUM
ESCHERICHIA COLI
INDIUM 111
LUNGS
NEUTROPHILS
ANIMAL TISSUES
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INDIUM ISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MATERIALS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RESPIRATORY SYSTEM
TISSUES
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