Activity testing of alveolar macrophages and changes in surfactant phospholipids after irradiation in bronchoalveolar lavage: Experimental and clinical data

Journal Article · Wed Jul 01 04:00:00 EDT 1992 · Environmental Health Perspectives; (United States)

DOI:https://doi.org/10.1289/ehp.9297171· OSTI ID:6852163

Steinberg, F; Rehn, B; Kraus, R; Quabeck, K; Bruch, J; Beelen, D W; Schaefer, U W; Streffer, C ^[1]

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1. Univ. Clinics, Essen (Germany)

This study presents results of bronchoalveolar lavage (BAL) after irradiation to the lungs in mice as well as clinical data. The number of BAL cells, mainly macrophages, lymphocytes, and granulocytes, changed in a time-dependent manner. The phagocytic activity of the macrophages measured as the phagocytosis of microbeads and measured as the esterase activity also showed a strong time-dependent increase during the acute phase up to 21 days after irradiation. The contents of surfactant phospholipids (SF) and sphingomyelin (SPH; as a parameter for cell death) were quantified by HPLC. Both were significantly changed between day 2 and 21 after irradiation. Three BALs of a patient with idiopathic interstitial pneumonitis, who had received an allogenic bone marrow graft after total body irradiation with 10 Gy, showed similar effects in the cellular and surfactant parameters. These data indicate that there are positive interactions between the number of different BAL cells, macrophage activity, and SF and SPH content in the preclinical model of the mouse as well as in the clinical situation after lung irradiation. 30 refs., 7 figs., 3 tabs.

• OSTI ID: 6852163
• Journal Information: Environmental Health Perspectives; (United States), Journal Name: Environmental Health Perspectives; (United States) Vol. 97; ISSN 0091-6765; ISSN EVHPAZ
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550603* -- Medicine-- External Radiation in Therapy-- (1980-)
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
ESTERS
IRRADIATION
LEUKOCYTES
LIPIDS
LUNGS
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHAGOCYTES
PHAGOCYTOSIS
PHOSPHOLIPIDS
RADIATION EFFECTS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
TIME DEPENDENCE
VERTEBRATES