Effects of dietary fat and calorie on immunologic function
The effect of dietary fat and calories on immunologic function in specific pathogen-free inbred DBA/2 and CBA/J mice was studied. Three diets were modified from control, the AIN-76 purified diet. The high saturated fat diet contained 22.5% coconut oil and 2.5% safflower oil. The high unsaturated fat diet contained 25% safflower oil. Fat was substituted isoclorically for carbohydrate in these two diets. The low calorie diet contained 40% less protein, carbohydrate and fat than control diet; fiber was substituted for these ingredients. Female weanling mice were on the diets for more than 35 days before testing. The natural killer (NK) activity of spleen cells was determined by in vitro cytolysis of /sup 51/Cr-labeled YAC-1 cells. The spleen cells response to sheep red blood cells (SRBC) or allogeneic tumor EL-4 cells was measured after immunizing the mice with SRBC or EL-4 cells for 4 or 11 days, respectively. The results showed no significant effect of the low calorie diet on NK activity, anti-SRBC or anti-EL-4 response compared to normal diet. Anti-SRBC plaque response was significantly enhanced (27% higher), while anti-EL-4 response was significantly suppressed (15% less) with high saturated fat diet. NK activity was normal. Mice on high unsaturated fat diet showed suppressed anti-SRBC response (16% less) and anti-EL-4 response (17% less), while NK activity was significantly enhanced (70% higher).
- Research Organization:
- Univ. South Florida, Tampa
- OSTI ID:
- 6851329
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DIET
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
EVEN-ODD NUCLEI
FATS
IMMUNE REACTIONS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NUCLEI
RADIOISOTOPES
RODENTS
RUMINANTS
SHEEP
SOMATIC CELLS
SPLEEN CELLS
TRACER TECHNIQUES
VERTEBRATES