Lack of correlation between immunologic markers and cell surface ultrastructure in the leukemic phase of lymphoproliferative diseases

Conference · Sat Jan 01 04:00:00 EST 1977

OSTI ID:6850702

Golomb, Harvey M.; Simon, Deberah

In a prospective study of malignant cells from 13 patients with the leukemic phase of lymphoproliferative diseases, we wished to determine whether any correlation between the immunologic markers and the cell surface ultrastructure. Five patients had chronic lymphocytic leukemia, four had malignant lymphomas, poorly differentiated lymphocytic type, two had the Sezary syndrome, and one each had acute prolymphocytic leukemia and acute lymphocytic leukemia. Cell separation and isolation was done at room temperature for all specimens. Immunologic markers tested for were surface immunoglobins, a B-cell property, and E-rosettes, a T-cell property. Three patients had T-cell diseases, 6 had B-cell diseases, and 4 were classified as ''null.'' All but one patient had moderate to large numbers of microvilli on their malignant cells. The single exception had a typical B-cell form of chronic lymphocytic leukemia. There appears to be no correlation between immunologic markers and cell surface ultrastructure; therefore, SEM appears not to be valuable in the diagnosis or classification of immunologic sub-types of certain lymphoproliferative diseases.

• Research Organization: Chicago Univ., Ill. (USA). Dept. of Medicine; Franklin McLean Memorial Research Inst., Chicago, Ill. (USA)
• Sponsoring Organization: USDOE
• OSTI ID: 6850702
• Report Number(s): CONF-770333--8
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTRON MICROSCOPY
HEMIC DISEASES
IMMUNE REACTIONS
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
MICROSCOPY
MORPHOLOGICAL CHANGES
NEOPLASMS
PATIENTS
SOMATIC CELLS
THYMOCYTES
TUMOR CELLS
ULTRASTRUCTURAL CHANGES