Hematopoiesis on cellulose ester membranes (CEM). X. Effects of in vitro irradiation of stromal cells prior to application on CEM
Journal Article
·
· Exp. Hematol.; (United States)
OSTI ID:6847468
Cellulose ester membranes (CEM) were coated with stromal cells from murine bone or bone marrow irradiated in vitro with 1000, 2000, or 4000 rad and then implanted i.p. in CAF1 mice for periods of six and 12 months. CEM coated with stromal cells from bone showed excellent regeneration of bone and hematopoiesis after 1000 rad in vitro irradiation. After 2000 rad, hematopoietic and bone regeneration was reduced by about 50%, and after 4000 rad it was completely absent in CEM coated with stromal cells from bone. CEM coated with stromal cells from bone marrow showed no regeneration of hematopoiesis or bone after 1000, 2000, and 4000 rad in vitro irradiation and residence i.p. for six and 12 months. These results indicate that regeneration of the hematopoietic microenvironment is dependent upon living stromal cells. A difference in radiation sensitivity is demonstrated between stromal cells from bone and from bone marrow.
- Research Organization:
- Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL
- OSTI ID:
- 6847468
- Journal Information:
- Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 10; ISSN EXHMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BLOOD FORMATION
BODY
BONE CELLS
BONE MARROW
BONE MARROW CELLS
CARBOHYDRATES
CELLULOSE
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
HEMATOPOIETIC SYSTEM
IN VITRO
MAMMALS
MEMBRANES
MICE
ORGANIC COMPOUNDS
ORGANS
PERITONEUM
POLYSACCHARIDES
RADIATION EFFECTS
RADIOSENSITIVITY
RECOVERY
RODENTS
SACCHARIDES
SEROUS MEMBRANES
SOMATIC CELLS
TISSUES
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BLOOD FORMATION
BODY
BONE CELLS
BONE MARROW
BONE MARROW CELLS
CARBOHYDRATES
CELLULOSE
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
HEMATOPOIETIC SYSTEM
IN VITRO
MAMMALS
MEMBRANES
MICE
ORGANIC COMPOUNDS
ORGANS
PERITONEUM
POLYSACCHARIDES
RADIATION EFFECTS
RADIOSENSITIVITY
RECOVERY
RODENTS
SACCHARIDES
SEROUS MEMBRANES
SOMATIC CELLS
TISSUES
VERTEBRATES