Radioenzymatic assay for quinolinic acid
Journal Article
·
· Anal. Biochem.; (United States)
A new and rapid method for the determination of the excitotoxic tryptophan metabolite quinolinic acid is based on its enzymatic conversion to nicotinic acid mononucleotide and, in a second step utilizing (/sup 3/H)ATP, further to (/sup 3/H) deamido-NAD. Specificity of the assay is assured by using a highly purified preparation of the specific quinolinic acid-catabolizing enzyme, quinolinic acid phosphoribosyltransferase, in the initial step. The limit of sensitivity was found to be 2.5 pmol of quinolinic acid, sufficient to conveniently determine quinolinic acid levels in small volumes of human urine and blood plasma.
- Research Organization:
- Maryland Psychiatric Research Center, Baltimore
- OSTI ID:
- 6846673
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AROMATICS
ATP
AZAARENES
AZINES
AZOLES
CARBOXYLIC ACIDS
COENZYMES
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLITES
NAD
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PYRIDINES
PYRROLES
RADIOENZYMATIC ASSAY
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
TRYPTOPHAN
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AROMATICS
ATP
AZAARENES
AZINES
AZOLES
CARBOXYLIC ACIDS
COENZYMES
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLITES
NAD
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PYRIDINES
PYRROLES
RADIOENZYMATIC ASSAY
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
TRYPTOPHAN