Wheat germ lectin-Sepharose affinity adsorption assay for the soluble glucagon receptor
An assay was developed based on the observation that many hormone receptors are glycoproteins. To test if the glucagon receptor is a glycoprotein, the receptor was used that had (/sup 125/I-Tyr/sup 10/)monoiodoglucagon covalently attached. The covalently labelled receptor was solubilized and exposed to wheat germ lectin-Sepharose in the presence and absence of various sugars. The sugar specificity for the adsorption of the glucagon receptor indicated that the receptor is a glycoprotein. The primary structure of glucagon is known and has been shown that it has no sugars attached to it. Therefore, the different in covalently attached sugars between the hormone and the receptor was used to develop an assay for the solubilized receptor. The hormone-receptor complex was specifically adsorbed onto the lectin-Sepharose while the free hormone remained in solution.
- OSTI ID:
- 6846114
- Journal Information:
- Methods Enzymol.; (United States), Vol. 109
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCAGON
RADIORECEPTOR ASSAY
ADSORPTION
EXPERIMENTAL DATA
GLUCOPROTEINS
IODINE 125
LABELLED COMPOUNDS
SACCHARIDES
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SORPTION
550201* - Biochemistry- Tracer Techniques