Vitro packaging of bacteriophage T7 DNA requires ATP. [Escherichia Coli]
Journal Article
·
· J. Virol.; (United States)
OSTI ID:6844874
Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 6844874
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 43:1; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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