Effect of oxygen transfer rate on the composition of the pectolytic enzyme complex of Aspergillus niger

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260231007· OSTI ID:6842984

Zetelaki-Horvath, K; Vas, K

Optimal agitation and aeration conditions (assuring O/sub 2/ transfer rates (OTR) of 12-179 mmol/L-h) were determined for pectin lyase (PL) synthesis of an Aspergillus niger strain. Components of the pectolytic enzyme complex were also investigated in order to determine whether their O/sub 2/ demand is identical with or different from that of pectin lyase. Should the latter be the case, a possibility would be given to produce enzyme complexes of different agitation and aeration conditions. The mycelium yield of Aspergillus niger was maximum at an OTR of 100 mmol/L-h. The yields of the various pectolytic enzymes reached maximum at different OTRs. PL production was highest (0.555 mumol/min-mL) at an OTR of 60 mmol/L-h. Endopolygalacturonase (PG) production has a maximum at OTR 49 mmol/L-h, with a 2nd peak at 100-135 mmol O2/L-h. Pectin esterase (PE) synthesis showed a maximum at an OTR of 12-14 mmol/L-h, while both apple juice clarifying and macerating activities gave 2 maximum at 14 and 60 mmol/L-h due to the optima of PE and endo-PG. Macerating activity showed a high value at OTR optimal for PL production as well.

• Research Organization: Cent. Food Res. Inst., Budapest, Hungary
• OSTI ID: 6842984
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 23:10; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AERATION
ASPERGILLUS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BLOOD SUBSTITUTES
CARBOHYDRATES
DRUGS
ELEMENTS
ENZYMES
FUNGI
HEMATOLOGIC AGENTS
KINETICS
LYASES
NONMETALS
ORGANIC COMPOUNDS
OXYGEN
PECTINS
PLANTS
POLYSACCHARIDES
REACTION KINETICS
SACCHARIDES
STIRRING
SYNTHESIS