Viscosity of the internal aqueous phase of unilamellar phospholipid vesicles

Journal Article · Tue Jul 01 00:00:00 EDT 1980 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(80)90473-7· OSTI ID:6839786

Clement, N R; Gould, J M

The viscosity of the internal aqueous phase of unilamellar vesicles comprised of purified soybean phospholipids (asolectin) was measured using the fluorescence polarization of the entrapped hydrophilic probe 8-hydroxy-1, 3, 6-pyrenetrisulfonate (pyranine). At 20/sup 0/C the rotational relaxation time (rho) for pyranine in bulk solution was 0.55 ns as compared to a rotational time of 1.8 ns for pyranine within the internal aqueous compartment. Similar large increases in rho for internal pyranine were noted over the temperature range 5 to 35/sup 0/C suggesting that in these small vesicles the internal water is more viscous than bulk water.

• Research Organization: Univ. of Notre Dame, IN
• OSTI ID: 6839786
• Journal Information: Arch. Biochem. Biophys.; (United States), Vol. 202:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AQUEOUS SOLUTIONS
VISCOSITY
LAMELLAE
PYRANS
FLUORESCENCE
POLARIZATION
RELAXATION
TEMPERATURE DEPENDENCE
MEMBRANES
PHOSPHOLIPIDS
DISPERSIONS
ESTERS
HETEROCYCLIC COMPOUNDS
LIPIDS
LUMINESCENCE
MIXTURES
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
SOLUTIONS
550200* - Biochemistry