Attachment of UDP-hexosamines to the ribosomes isolated from rat liver

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Biochem. Med. Metab. Biol.; (United States)

DOI:https://doi.org/10.1016/0885-4505(88)90088-6· OSTI ID:6838441

Kopacz-Jodczyk, T; Paszkiewicz-Gadek, A; Galasinski, W

The binding of UDP-N-acetylhexosamines with purified ribosomes was studied and it was found that the radioactive nucleotides can be attached to these particles. The radioactivity of the purified ribosomal pellet depends on the amounts of ribosomes and UDP-N-acetylhexosamines. Some characteristics of the binding system indicate that the attachment of UDP-sugar to ribosome does not require the participation of glycosyltransferases. The results of the competition experiment would suggest that there are specific sites on ribosomes for the binding of UDP-N-acetylglucosamine.

• Research Organization: Institute of Chemistry, Bialystok (Poland)
• OSTI ID: 6838441
• Journal Information: Biochem. Med. Metab. Biol.; (United States), Vol. 39:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HEXOSAMINES
BIOCHEMICAL REACTION KINETICS
GLYCOSYL TRANSFERASES
LIVER
RADIOISOTOPES
RATS
RIBOSOMES
TRACER TECHNIQUES
AMINES
ANIMALS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
REACTION KINETICS
RODENTS
SACCHARIDES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques