H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen

Journal Article · Sun May 15 04:00:00 EDT 1988 · J. Biol. Chem.; (United States)

OSTI ID:6838377

Collier, I E; Wilhelm, S M; Eisen, A Z; Marmer, B L; Grant, G A; Seltzer, J L; Kronberger, A; He, C; Bauer, E A; Goldberg, G I

H-ras transformed human bronchial epithelial cells (TBE-1) secrete a single major extracellular matrix metalloprotease which is not found in the normal parental cells. The enzyme is secreted in a latent form which can be activated to catalyze the cleavage of the basement membrane macromolecule type IV collagen. The substrates in their order of preference are: gelatin, type IV collagen, type V collagen, fibronectin, and type VII collagen; but the enzyme does not cleave the interstitial collagens or laminin. This protease is identical to gelatinase isolated from normal human skin explants, normal human skin fibroblasts, and SV40-transformed human lung fibroblasts. Based on this ability to initiate the degradation of type IV collagen in a pepsin-resistant portion of the molecule, it will be referred to as type IV collagenase. This enzyme is most likely the human analog of type IV collagenase detected in several rodent tumors. Type IV collagenase consists of three domains. Type IV collagenase represents the third member of a newly recognized gene family coding for secreted extracellular matrix metalloproteases, which includes interstitial fibroblast collagenase and stromelysin.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO (USA)
• OSTI ID: 6838377
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:14; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOCHEMISTRY
BIODEGRADATION
BODY
BRONCHI
CARBON 14 COMPOUNDS
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
CHEMISTRY
COLLAGEN
CONNECTIVE TISSUE CELLS
DECOMPOSITION
ENZYMES
EPITHELIUM
FIBROBLASTS
GENES
HYDROLASES
LABELLED COMPOUNDS
MAMMALS
MAN
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
SCLEROPROTEINS
SKIN
SOMATIC CELLS
TISSUES
VERTEBRATES