Nuclear magnetic resonance studies of intracellular pH and pH homeostasis in the hog carotid artery

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6834328

Grieder, T A

Intracellular pH (pH{sub i}) is an important determinant of vascular smooth muscle (VSM) contractility and relaxation. Most NMR measurement of pH have been calculated from the chemical shift of inorganic phosphate (P{sub i}) in {sup 31}P spectra. An alternative approach is to calculate pH from the difference in chemical shifts of signals in the {sup 19}F spectrum of cells loaded with difluoromethylalanine. This technique has higher sensitivity to pH changes and provides better time resolution than other NMR methods. In this study we report simultaneous measurements of pH{sub i} and the contractile state of single, intact hog carotid arterial segments, closed at both ends and superfused with HCO{sub 3}{sup {minus}}-buffered Krebs solution at physiological pressures. At 28{degree}C, resting arteries maintained a pH{sub i} of 7.15 {+-} 0.03 units (n = 16). In a parallel study, helically cut strips studied with {sup 31}P NMR maintained a similar resting pH (7.18 {+-} 0.09).

• Research Organization: University of Medicine and Dentistry of New Jersey, Piscataway, NJ (USA). Rutgers Medical School
• OSTI ID: 6834328
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MUSCLES
CONTRACTION
CHEMICAL SHIFT
HOMEOSTASIS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PH VALUE
PHOSPHORUS 31
SWINE
TRACER TECHNIQUES
ANIMALS
DOMESTIC ANIMALS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
PHOSPHORUS ISOTOPES
RESONANCE
SPECTRA
STABLE ISOTOPES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques