Insulin receptor in Drosophila melanogaster
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6832530
A specific, high affinity insulin receptor is present in both adult Drosophila and in Drosophila embryos. Wheat germ lectin-enriched extracts of detergent-solubilized membranes from embryos and adults bind insulin with a K/sub d/ of 15 nM. Binding is specific for insulin; micromolar concentrations of proinsulin, IGFI, and IGFII are required to displace bound /sup 125/I-insulin. Insulin-dependent protein tyrosine kinase activity appears during embryogenesis. It is evident between 6 and 12 hours of development, peaks between 12 and 18 hours and falls in the adult. During 0-6 hours of embryogenesis, and in the adult, a specific protein band (Mr = 135,000) is crosslinked to /sup 125/I-insulin. During 6-12 and 12-18 hours of embryogenesis stages in which insulin-dependent protein tyrosine kinase is high, an additional band (Mr = 100,000) becomes crosslinked to /sup 125/I-insulin. Isolation and DNA sequence analysis of genomic clones encoding the Drosophila insulin receptor will be presented as will the characterization of insulin receptor mRNA's during development.
- Research Organization:
- Memorial Sloan-Kettering Cancer Center, New York, NY
- OSTI ID:
- 6832530
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CHEMICAL REACTIONS
CHEMISTRY
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DIPTERA
DNA SEQUENCING
DROSOPHILA
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
FLIES
FRUIT FLIES
HORMONES
INSECTS
INSULIN
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYMERIZATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
TRANSFERASES
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CHEMICAL REACTIONS
CHEMISTRY
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DIPTERA
DNA SEQUENCING
DROSOPHILA
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
FLIES
FRUIT FLIES
HORMONES
INSECTS
INSULIN
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYMERIZATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
TRANSFERASES