Insulin stimulates the tyrosine phosphorylation of a Mr = 160,000 glycoprotein in adipocyte plasma membranes

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6832524

Yu, K T; Khalaf, N; Czech, M P

In an attempt to identify putative substrates for the insulin receptor kinase, adipocyte plasma membranes were incubated with (..gamma..-/sup 32/P)ATP in the presence and absence of insulin. Insulin stimulates the tyrosine phosphorylation of its receptor ..beta.. subunit but does not detectably alter the phosphorylation of other membrane proteins. In contrast, when plasma membranes from insulin-treated adipocytes are phosphorylated, the /sup 32/P-labeling of a Mr=160,000 species (p160) and insulin receptor ..beta.. subunit are markedly increased when compared to controls. p160 exhibits a rapid response (max. at 1 min) and high sensitivity (ED/sub 50/ = 2 x 10/sup -10/M) to insulin. The stimulatory effect of insulin on the phosphorylation of p160 is rapidly reversed following the addition of anti-insulin serum. Cold chase experiments indicate that insulin promotes the phosphorylation of p160 rather than inhibiting its dephosphorylation. p160 is a glycoprotein as evidenced by its adsorption to immobilized lectins and does not represent the insulin receptor precursor. The action of insulin on p160 tyrosine phosphorylation is mimicked by concanavalin A but not by EGF and other insulin-like agents such as hydrogen peroxide and vanadate. These results suggest that p160 tyrosine phosphorylation is an insulin receptor-mediated event and may participate in signalling by the insulin receptor.

• Research Organization: Univ. of Massachusetts Medical School, Worcester
• OSTI ID: 6832524
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
AMINO ACIDS
ANIMAL CELLS
ANTIBODIES
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CONCANAVALIN
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYME REACTIVATION
ENZYMES
FAT CELLS
FUNCTIONS
GLUCOPROTEINS
HEMAGGLUTININS
HORMONES
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROXY ACIDS
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LECTINS
LIGHT NUCLEI
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PEROXIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
TYROSINE
VANADATES
VANADIUM COMPOUNDS