Mutagenicity of transformation metabolites of selected polycyclic aromatic hydrocarbons

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6832134

Paige, Jr, W E

The purpose of this study is: (1) to determine the efficacy of a microbial enhanced soil system in the degradation of selected polycyclic aromatic hydrocarbons (PAHs); (2) to determine if predictions based upon physical and chemical properties with respect to degree of recalcitrancy are borne out by observations in a laboratory environment; and (3) to determine the mutagenic potential of the degradation products by the Ames Mutagenicity Bioassay test. The two phase study examined the behavior of fluorene, benzo(b) fluoranthene, benzo(k) fluoranthene and dibenzo (a,h) anthracene in an enhanced soil system. The initial phase uses GC/MS analysis of both soil and water systems to determine if the PAHs had decreased in concentration. The final phase uses the Ames Bioassay to measure genotoxic potential. The results of the study suggest that benzo(k) fluoranthene produces mutagenic breakdown metabolites during microbial degradation and that volatilization is the significant loss mechanism for fluorene. Dibenzo(a,h) anthracene and benzo(b) fluoranthene are recalcitrant under the experimental conditions.

• Research Organization: North Carolina State Univ., Raleigh, NC (USA)
• OSTI ID: 6832134
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BENZANTHRACENE
BIODEGRADATION
CHEMICAL PROPERTIES
CHEMICAL REACTIONS
CHROMATOGRAPHY
CONDENSED AROMATICS
DECOMPOSITION
FLUORENE
GAS CHROMATOGRAPHY
HYDROCARBONS
MASS SPECTROSCOPY
METABOLIC ACTIVATION
METABOLITES
MUTAGEN SCREENING
ORGANIC COMPOUNDS
PHYSICAL PROPERTIES
POLYCYCLIC AROMATIC HYDROCARBONS
SCREENING
SEPARATION PROCESSES
SOILS
SPECTROSCOPY