Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions
A temperature-sensitive (ts) mutant, designated tsFT210, was isolated from a mouse mammary carcinoma cell line, FM3A. The tsFT210 cells grew normally at 33/sup 0/C (permissive temperature), but more than 80% of the cells were arrested at the G2 phase at 39/sup 0/C (non-permissive temperature) as revealed by flow-microfluorimetric analysis. DNA replication and synthesis of other macromolecules by this mutant seemed to be normal at 39/sup 0/C for at least 10h. However, in this mutant, hyperphosphorylation of H1 histone from the G2 to M phase, which occurs in the normal cell cycle, could not be detected at the non-permissive temperature. This suggests that a gene product which is temperature-sensitive in tsFT210 cells is necessary for hyperphosphorylation of H1 histone and that this gene product may be related to chromosome condensation.
- Research Organization:
- Univ. of Tokyo, Japan
- OSTI ID:
- 6831731
- Journal Information:
- Exp. Cell Res.; (United States), Vol. 167:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HISTONES
PHOSPHORYLATION
MUTANTS
TEMPERATURE DEPENDENCE
TRITIUM COMPOUNDS
TRACER TECHNIQUES
TUMOR CELLS
GENETICS
BIOSYNTHESIS
CARCINOMAS
CELL CYCLE
CELL FLOW SYSTEMS
CHROMOSOMES
DNA REPLICATION
MAMMARY GLANDS
MICE
ANIMAL CELLS
ANIMALS
BIOLOGY
BODY
CHEMICAL REACTIONS
DISEASES
GLANDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NEOPLASMS
NUCLEIC ACID REPLICATION
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
SYNTHESIS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques
550401 - Genetics- Tracer Techniques